Rajeshkumar et al. World Journal of Pharmacy and Pharmaceutical Sciences. ANTICANCER ACTIVITY OF ANDROGRAPHIS PANICULATA. showing anticancer activity on HEp2 – Human larynx carcinoma cell lines in-vitro KEYWORDS: Andrographis paniculata,Herbal medicine. A refined extract of A. paniculata, also significantly reduces activities of lipid .. The anticancer activity was observed due to the presence of andrographolide.

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BioMed Research International

Phenotypic correlation coefficient r among morphological and phytochemical traits in A. Calibration curve was generated by linear regression based on peak areas [ 42 ]. Accession showed a growth reduction of dry weight up to According to Talei et al. The reduction in sensitive accessions in terms of all studied morphological traits was higher than in tolerant panicu,ata Figure 1.

ME at the ratio of 1: The slight differences in retention times RT of the phytochemicals are due to the running of the samples in different batches using a refreshed mobile phase. Gour Central University, Sagar M.

Van Der Hejden, H. Therefore, the stress conditions have a strong impact on the responsible metabolic pathways for the accumulation of the related natural products [ 11 ].

Bounded stain was solubilised with Tris buffer 10 mM and the optical densities were read on an automated spectrophotometric plate reader Molecular Devices, USA at a wavelength of nm, Optical density OD of SRB in each well is directly proportional to the cell number.

All chemicals used were of analytical grade. To the best of our reference, the only conducted research related to salt stress in A.

So now a days they are widely used for curing many diseases including cancer. The dry weight was used to monitor the physiological response of each accession to salinity. Enhancement of phenolic and flavonoid compounds in onion plant under salinity stress has been reported to improve the deleterious effect of salinity stress [ 34 anticacer. Hepatoprotective and antioxidant property of Andrographis paniculata Nees in BHC induced liver damage in mice. The dried materials were chopped into small pieces and ground into fine powder form and were then extracted with a mixture ativity dichloromethane and methanol DCM: Our results indicated a positive correlation between the three anticancer phytochemicals and proline accumulation, which might be closely related to tolerance abilities indicated by physiological performances.


Since metabolic adaptations towards environmental stress acticity salinity affect global metabolic fluxes, some beneficial secondary metabolites of therapeutic importance or so-called phytochemicals would also be affected [ 11 ]. In other words, the increase of these phytochemicals under salinity condition would be partially compensated by a decline in total biomass.

However, improving the content of some active compounds such as flavonoids could be achieved under abiotic stress condition as an agronomical approach [ 1214 ] and biotechnological approaches genetic transformation [ 15 ] on a large scale in plants.

Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.

Subscribe to Table of Contents Alerts. Increases in certain amino acids including prolinesugars including sucrose, fructose, and glucoseand polyols including inositols have been reported in some plant species like grapevine [ 7 ], Limonium latifolium [ 8 ], and Lotus japonicas [ 9 ].

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Many of those metabolic changes such as sucrose, oligosaccharides, polyhydric alcohols, proline, and polyamines are observable in the leaves of plants exposed to abiotic stresses [ 4 ]. These natural products are supposed to minimize DNA damage by reacting with free radical s and in this way they can andrographks cancer.

Variations due to salinity levels SL and accessions AC were highly significant. Anticancer activity of hydroalcoholic extract of A. Journal of Pharmacology and Activitu, 8: How to cite this article: Each plant was irrigated once a day with five levels of saline water.

The correlative aspects of the three anticancer phytochemicals production as well as the accumulation of proline in the tolerant and sensitive A. The authors of the paper declare that they do not have any direct financial relation with the commercial identities mentioned in the present paper that might lead to conflict of interests for any of the authors.

View at Google Scholar A. The following drugs and chemicals were used: Vertical bars represent standard error of mean for three samples.


Analysis of the Anticancer Phytochemicals in Andrographis paniculata Nees. under Salinity Stress

This can be utilized as a potential in the next breeding programs to develop the salt-tolerant varieties in A. Abstract Andrographis paniculata and Silybum marianum are well known medicinal plants.

The isocratic mobile phase was prepared with acetonitrile water Previous studies have demonstrated that the actifity of A. Several herbs and plants with diversified pharmacological properties have been shown to have potential to prevent human cancers as they possess antitumor substances which have the potential to cure cancer without causing toxicity.

The results indicated that salinity anddrographis a significant effect on the aforementioned three anticancer phytochemicals.

Reportedly, improving the content of andgographis phytochemicals such as flavonoids as one of the most important pharmaceutical contents could be enhanced through salinity and drought stresses [ 121415 ]. However, the standard curves were compared for each sample and the reliability of the peaks was confirmed accordingly. So, here we are using the natural drugs from phytochemicals to reduce the toxicity level.

The process was repeated several times with the same solvent system until the solvent portion becomes colorless. Effects of salinity levels on TDW, proline and three main phytochemicals in tolerant and sensitive accession of A.

Under the low level of salinity, the salt ions inhibited the biosynthesis of phytochemicals as indicated by the decrease in their amounts in both tolerant and sensitive accessions. This is an open access article distributed under the Creative Commons Attribution Licensewhich permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Culturing of cell line: Percentage inhibition was calculated by comparing the OD of control well with that of test panicu,ata. These compounds are produced mainly from aerial organs of the plant, especially leaves. Protective effects of silymarin against photocarcinogenesis in a mouse skin model.