Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].

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For scanning semijis microscopy, cultured A. Analysis of the protein profiles and outer membrane antigens of A. Polyclonal serum anti- A. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Looking for a protein specific to this species, we determined the protein profile of subcellular fractions of A. Production and preliminary characterization of monoclonal antibodies to Actinobacillus sp. A single, near term lamb with a crown rump length of 40cm was necropsied.

Additionally, in the case of H. Mild multifocal lymphohistiocytic actinlbacillus was observed in srminis and submucosa of the urinary bladder. The main causative agents of ovine infectious epididymitis are Brucella ovisActinobacillus seminisand Histophilus somni [ 1 — 3 ]. The length and width of the left actinobacilljs tails of rams H. B, extract from A. The first isolation was reported from Australia in by Baynes and Simmons 6who described suppurative epididymitis caused by a gram-negative pleomorphic microorganism in a ram.

We also isolated and identified this protein in microvesicles released to the culture supernatant. Immunohistochemical technique used was only effective for immunolabeling A. It was confirmed that A.


Actinobacillus seminis – Wikipedia

Testicular parameters of rams experimentally infected with Actinobacillus seminis aeminis Histophilus somni during 42 days of infection.

Ovine brucellosis caused by B. View at Google Scholar I. Infectious epididymitis in rams is a major disease in countries devoted sejinis the industrialized breeding of sheep. Then ductal rupture can occur with extravasation of sperm, followed by diffuse fibrosis and granulomatous inflammation and formation of spermatic granulomas, as reported in this and other studies [ 81216 ].

Subcellular fractions of A. Atinobacillus isolate was PCR positive for A. In the Australian context, epididymitis is the most important manifestation of A. Left testis is decreased in volume and tunica vaginalis is thickened and firmly adhered to the testis. Gram-negative bacteria form microvesicles, or blebs, as a result of outer membrane budding.

A pattern corresponding to smooth LPS was found in A. The vesicle diameter varied from 20 to nm, as was described for A.

The actinobcaillus of our study was to identify a protein specific to A. There is a single report of A. To avoid cross-contamination between semen samples from different rams in each group, we used a sterile, disposable plastic inside the artificial vagina, connected directly to collection tube. While some Australian flocks may have attempted to eradicate A.

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Length a and width b of the tail of the epididymides of rams experimentally infected with Actinobacillus seminis or Histophilus somni during the course of infections. Electron microscopy For negative staining, cultured A. Results Prior to inoculation, all 20 rams were clinically healthy, without palpable lesions in the testes or epididymides. Ovine infectious epididymitis is one of the major causes of reproductive disorders in sheep, and actinobacilous strongly impacts on the productivity of the herds [ 1 ].


Identification and characterization of microorganisms has historically been based on morphologic, biochemical, actinobaccillus, toxigenic, and genetic characteristics. Although both agents are found in the preputial flora of healthy young sheep [ 5 ], A.

In addition, frequency of detection of A.

Gross and microscopic findings in both experimental infections were similar to those seen in actnobacillus of natural infection [ 3101124 — 26 ]. The rams were divided into two groups of 10 animals each and the experiments were carried out in Belo Horizonte, Brazil Traduit par Docteur Serge Messier. Microscopically, the main lesions were inflammatory changes in the genitourinary tract and testicular seminls.

Similar changes have been observed in the testes of rams experimentally infected with B.

To determine which proteins of A. These antigenic protein profiles provide the first data, to our knowledge, regarding various subcellular fractions of A.

The results suggest that the kDa protein could be used to establish a diagnostic test specific for ovine epididymitis caused by A.

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Several previous studies evaluated the pathological changes in rams experimentally infected with B. View at Google Scholar R. To determine if the kDa protein was species-specific for A. Introduction Actinoabcillus infectious epididymitis is one of the major causes of reproductive disorders in sheep, and it strongly impacts on the productivity of the herds [ 1 ]. Am J Vet Res.